The CAD risk locus 9p21 increases the risk of vascular calcification in an iPSC-derived VSMC model

Abstract Background Coronary artery disease (CAD) is the leading cause of death worldwide. Chromosome locus 9p21 was first to be associated with increased risk CAD and coronary calcification (CAC). Vascular increases for CAD. smooth muscle cells (VSMCs) are one major cell types involved in development vascular calcification. Methods So far, mainly animal models or primary SMCs have been used model human In this study, a vitro assay using iPSC-derived VSMCs developed examine Human iPSCs were derived from healthy non-risk (NR) (R) donor carrying SNPs locus. Additionally, knockouts each iPSC line (NR R) used. Following differentiation, characterized based on type, proliferation, migration rate, along calcium phosphate (CaP) deposits. CaP deposits confirmed Calcein Alizarin Red S staining then quantified. Results The data demonstrated significantly more migration, deposition R both KO lines than those NR line. Molecular analyses upregulation markers. These results consistent recent demonstrating when murine ortholog knocked-out. Conclusion Therefore, conclusion, genetic variation deletion leads an This could develop new drug screening strategies combat CAC.